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yeast expression vector pact2  (TaKaRa)


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    TaKaRa yeast expression vector pact2
    Yeast Expression Vector Pact2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pact2+yeast+expression+vector/pm33798946-46-13-17
    Average 86 stars, based on 1 article reviews
    yeast expression vector pact2 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: p63β modulates c-Myc activity via direct interaction and regulation of MM1 protein stability
    Article Snippet: .. The MATCHMAKER GAL4 Two-Hybrid System 3 (Clontech) and a cDNA library derived from human mammary gland in the pACT2 yeast expression vector (Clontech) were used to screen interacting proteins of p63αCT according to the manufacturer's instructions. .. H1299, HeLa, 293T, A549, and MiaPaCa-2 cells were maintained in Dulbecco's modified Eagle's medium (DMEM, Hyclone) supplemented with 10% fetal bovine serum (FBS, Hyclone) and 1% penicillin/streptomycin (Hyclone).

    Derivative Assay:

    Article Title: p63β modulates c-Myc activity via direct interaction and regulation of MM1 protein stability
    Article Snippet: .. The MATCHMAKER GAL4 Two-Hybrid System 3 (Clontech) and a cDNA library derived from human mammary gland in the pACT2 yeast expression vector (Clontech) were used to screen interacting proteins of p63αCT according to the manufacturer's instructions. .. H1299, HeLa, 293T, A549, and MiaPaCa-2 cells were maintained in Dulbecco's modified Eagle's medium (DMEM, Hyclone) supplemented with 10% fetal bovine serum (FBS, Hyclone) and 1% penicillin/streptomycin (Hyclone).

    Expressing:

    Article Title: p63β modulates c-Myc activity via direct interaction and regulation of MM1 protein stability
    Article Snippet: .. The MATCHMAKER GAL4 Two-Hybrid System 3 (Clontech) and a cDNA library derived from human mammary gland in the pACT2 yeast expression vector (Clontech) were used to screen interacting proteins of p63αCT according to the manufacturer's instructions. .. H1299, HeLa, 293T, A549, and MiaPaCa-2 cells were maintained in Dulbecco's modified Eagle's medium (DMEM, Hyclone) supplemented with 10% fetal bovine serum (FBS, Hyclone) and 1% penicillin/streptomycin (Hyclone).

    Article Title: The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis
    Article Snippet: The purified PCR products were subcloned into the pGEM-T Easy vector (Promega, Madison, WI). .. The HPS4 cDNA inserts were excised from the vector with appropriate restriction enzymes and subcloned into the pEF-T7/FLAG tag mammalian expression vectors (46), pAct2 yeast expression vector (Clontech-Takara Bio), and/or pMRX-IRES-HA tag retrovirus vector (47) as described previously. .. The pHPS1 vector was obtained from the pGBDU-C1 yeast expression vector (48) by replacing the GAL4 sequence with the mouse HPS4 cDNA sequence. pGBD-C1-Rab(CA/CN) lacking the C-terminal geranylgeranylation site (ΔCys) except pGBD-C1-Rab6D/41ΔCys and pGBD-C1-Rab40ALΔCys were prepared as described previously (22–24). cDNAs encoding Rab6D(T44N/Q90L)ΔCys and Rab40AL(S28N/Q73L)ΔCys were produced by the two-step PCR techniques using human Rab6D cDNA and Rab40AL cDNA (49), respectively, as a template and subcloned into the pGBD-C1 vector (48).

    Plasmid Preparation:

    Article Title: p63β modulates c-Myc activity via direct interaction and regulation of MM1 protein stability
    Article Snippet: .. The MATCHMAKER GAL4 Two-Hybrid System 3 (Clontech) and a cDNA library derived from human mammary gland in the pACT2 yeast expression vector (Clontech) were used to screen interacting proteins of p63αCT according to the manufacturer's instructions. .. H1299, HeLa, 293T, A549, and MiaPaCa-2 cells were maintained in Dulbecco's modified Eagle's medium (DMEM, Hyclone) supplemented with 10% fetal bovine serum (FBS, Hyclone) and 1% penicillin/streptomycin (Hyclone).

    Article Title: The BLOC-3 subunit HPS4 is required for activation of Rab32/38 GTPases in melanogenesis, but its Rab9 activity is dispensable for melanogenesis
    Article Snippet: The purified PCR products were subcloned into the pGEM-T Easy vector (Promega, Madison, WI). .. The HPS4 cDNA inserts were excised from the vector with appropriate restriction enzymes and subcloned into the pEF-T7/FLAG tag mammalian expression vectors (46), pAct2 yeast expression vector (Clontech-Takara Bio), and/or pMRX-IRES-HA tag retrovirus vector (47) as described previously. .. The pHPS1 vector was obtained from the pGBDU-C1 yeast expression vector (48) by replacing the GAL4 sequence with the mouse HPS4 cDNA sequence. pGBD-C1-Rab(CA/CN) lacking the C-terminal geranylgeranylation site (ΔCys) except pGBD-C1-Rab6D/41ΔCys and pGBD-C1-Rab40ALΔCys were prepared as described previously (22–24). cDNAs encoding Rab6D(T44N/Q90L)ΔCys and Rab40AL(S28N/Q73L)ΔCys were produced by the two-step PCR techniques using human Rab6D cDNA and Rab40AL cDNA (49), respectively, as a template and subcloned into the pGBD-C1 vector (48).



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